The Montpellier lock was left less than impressed as South Africa capitalised on poor line-out play to stroll to a 28-0 win in Edinburgh last weekend.
Hamilton, who won his first cap in November 2006 against Romania, does not want a repeat showing against the Wallabies after a painful defeat to a ruthless South African team.
He said: “We were extremely down.
“We genuinely went into the game feeling that, if we got things right, we could go on and win.
“It was a very good South Africa team but it was there for all to see – we just did not get a foothold in the game.
“We lost five of the first six line-outs and they scored from two driving line-outs.
“With the runaway tries they got, it was fairly disappointing and we have to get over it quickly.”
Swindon-born Hamilton will team up with Tim Swinson in the second row at Murrayfield and has no plans to announce his international retirement in the near future despite ever-changing make-up of international squads.
He said: “I don’t feel too old yet. I played a few games with Nathan Hines. I might have played once with Scott Murray, a couple with Jason White maybe, then Richie Gray and big Al Kellock and the other guys in the mix now.
“It has always been about trying to work as hard as I possibly can and not taking my place for granted.
“Scotland has had some great second row players over the years and they have pushed me on to become better.
“It is a massive honour to win a 50th cap and a huge milestone for me to look back on – but it is one I want to be victorious in.
“Emotionally, it is going to be huge for me, but getting up for the game is never an issue.
“It is about making sure we get the details right and that is all done in training.”