Scottish voters appeared to have backed a Remain vote as polls closed last night.
All five of Holyrood’s party leaders had urged Scots to back staying in the EU, making an 11th hour proclamation on the eve of yesterday’s vote.
Polls had continuously suggested strong support for the Remain camp north of the border – and early indications appeared to show a majority rejected Brexit.
However, Leave activists were hoping to pick up support in areas such as the north-east, with one survey suggesting 92% of fishermen would back breaking with Brussels.
Last night, pro-EU campaigners from the ‘Scotland Stronger In’ campaign gathered in Glasgow to watch the results.
Ahead of the poll, the campaign delivered a final message to voters urging them to “take 20 minutes out of their time” to vote Remain and “make a lifetime difference for the better”.
Scottish Vote Leave activists meanwhile claimed the vote was “Scotland’s last chance to empower our parliament”.
Reports suggested turnout across Scotland was high, with good weather across much of the country helping tempt voters to the polls.
Around 56% of those eligible turned out to vote in May’s Holyrood election, but it is likely turnout at yesterday’s poll was higher.
However, it had been suggested a weariness of elections north of the border could see a lower turnout in Scotland than the rest of the UK. The EU referendum was the fourth national vote for Scots in less than two years.
Nicola Sturgeon cast her vote in Glasgow yesterday morning, while Ruth Davidson voted in her constituency of Edinburgh Central.
Ms Sturgeon had been actively campaigning for a Remain vote – but suggested Scotland being taken out of the EU against its will could trigger a second referendum on independence.
Scottish Labour leader Kezia Dugdale meanwhile took to the streets of Edinburgh’s Morningside with her party’s only MP north of the border, Ian Murray, in a bid to get out the vote for Remain.
Ms Dugdale had earlier voted alongside her partner, Louise Riddell, in the capital.
The leader of Scottish UKIP – and the party’s only elected representative north of the border – David Coburn, cast his vote in Kinghorn, Fife.
Polling stations across Scotland opened at 7am, with the ballot box for the Hebridean island of Gigha – which has 122 voters – having to be transported to the island by ferry on Tuesday. Polls closed at 10pm.